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The Dorsomedial Hypothalamic Nucleus Is Not Necessary for the Expression of Circadian Food-Anticipatory Activity in Rats

Department of Psychology, Simon Fraser University, Burnaby, BC, Canada

Glenn R. Yamakawa

Department of Psychology, Simon Fraser University, Burnaby, BC, Canada

Ian C. Webb

Department of Psychology, Simon Fraser University, Burnaby, BC, Canada

Rhiannon J. Mear

Department of Psychology, Simon Fraser University, Burnaby, BC, Canada

Ralph E. Mistlberger

Department of Psychology, Simon Fraser University, Burnaby, BC, Canada, mistlber{at}sfu.ca

Restricted daytime feeding generates food-anticipatory activity (FAA) by entrainment of a circadian pacemaker separate from the light-entrainable pacemaker located in the SCN. The dorsomedial hypothalamic nucleus (DMH) has been proposed as the site of food-entrainable oscillators critical for the expression of FAA, but another study found no effects of complete DMH ablation on FAA. To account for these different results, the authors examined methodological factors, including (1) cage configuration and feeding method and (2) use of social cues. Intact and DMH-ablated rats were maintained on one 4-h daily meal in the middle of the light period, using caging and feeding methods matching those of Gooley et al. (2006). Rats with partial or complete DMH ablation were less nocturnal during ad lib food access but exhibited normal FAA during restricted feeding, as quantified by FAA magnitude, ratios, latency to appearance, duration, and precision. To evaluate the use of social cues, intact rats naive to restricted-feeding schedules were food deprived for 72 h on 4 tests. Daytime activity increased during food deprivation, but the magnitude and waveform of this activity was not influenced by the presence of food-entrained rats exhibiting robust FAA in adjacent cages. Thus, hungry intact rats do not use social cues to anticipate a daily mealtime, suggesting that DMH-ablated rats do not anticipate meals by reacting to sounds from food-entrained intact rats in adjacent cabinets. These results confirm our previous finding that the DMH is not critical for normal expression of FAA in rats, and this observation is extended to food restriction methodologies used by other labs. The methodological differences that do underlie discrepant results remain unresolved, as does the location of food-entrainable oscillators, input pathways, and output pathways critical for FAA.

Key Words: circadian rhythms • food entrainment • locomotor activity • dorsomedial nucleus of hypothalamus • restricted feeding • rat

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Journal of Biological Rhythms, Vol. 22, No. 6, 467-478 (2007)
DOI: 10.1177/0748730407307804


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (9) Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Landry, G. J. Articles by Mistlberger, R. E. Search for Related Content PubMed PubMed Citation Articles by Landry, G. J. Articles by Mistlberger, R. E. Social Bookmarking                         What's this?