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Non-Ventral Lateral Neuron-Based, Non-PDF-Mediated Clocks Control Circadian Egg-Laying Rhythm in Drosophila melanogaster

Chronobiology Laboratory, Evolutionary and Organismal Biology Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India

Dhanashree A. Paranjpe

Chronobiology Laboratory, Evolutionary and Organismal Biology Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India

Vijay Kumar Sharma

Chronobiology Laboratory, Evolutionary and Organismal Biology Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India, vsharma{at}jncasr.ac.in

The authors report the results of their study aimed at investigating the consequence of targeted ablation of ventral lateral neurons (LN_vs—neurons regulating eclosion and locomotor activity rhythms) and genetic disruption of pigment-dispersing factor (PDF—an important output of circadian clocks) on the egg-laying rhythm of Drosophila melanogaster. The results clearly suggest that genetic ablation of LN_vs and loss of function mutation of PDF abolish eclosion and locomotor activity rhythms, whereas the egg-laying rhythm continues unabated. Furthermore, the results also demonstrate that the period of egg-laying rhythm remains unchanged under different ambient temperatures and nutrition levels, suggesting that the egg-laying rhythm of D. melanogaster is temperature and nutrition compensated. Based on these results, the authors conclude that the egg-laying rhythm in D. melanogaster is regulated by non-LN_v-based, non-PDF-mediated circadian clocks.

Key Words: Drosophila • egg laying • activity • eclosion • LNv • LNd • DN

References

• Blanchardon E, Grima B, Klarsfeld A, Chelot E, Hardin PE, Preat T, and Rouyer F (2001) Defining the role of Drosophila lateral neurons in the control of circadian activity and eclosion rhythms by targeted genetic ablation and PERIOD protein over expression. Eur J Neurosci 13:871-888.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Brand AH and Perrimon N (1993) Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118:401-415.[Abstract]
• Ewer J, Frisch B, Hamblen-Coyle MJ, Rosbash M, and Hall JC (1992) Expression of the period clock gene within different cell types in the brain of Drosophila adults and mosaic analysis of these cells’ influence on circadian behavioral rhythms. J Neurosci 12:3321-3341.[Abstract]
• Glossop NR and Shepherd D (1998) Disconnected mutants show disruption to the central projections of proprioceptive neurons in Drosophila melanogaster. J Neurobiol 36:337-347.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Han DD, Stein D, and Stevens LM (2000) Investigating the function of follicular subpopulations during Drosophilaoogenesis through hormone-dependent enhancer-targeted cell ablation. Development 127:573-583.[Abstract]
• Helfrich-Förster C (1998) Robust circadian rhythmicity of Drosophila melanogaster requires the presence of lateral neurons: A brain-behavioral study of disconnectedmutants. J Comp Physiol A 182:435-453.[CrossRef][Medline] [Order article via Infotrieve]
• Krishnan B, Dryer SE, and Hardin PE (1999) Circadian rhythms in olfactory response of Drosophila melanogaster. Nature 400:375-378.[CrossRef][Medline] [Order article via Infotrieve]
• Lin Y, Stormo GD, and Taghert PH (2004) The neuropeptide pigment-dispersing factor coordinates pacemaker interactions in the Drosophila circadian system. J Neurosci 24:7951-7957.[Abstract/Free Full Text]
• Mazzoni EO, Desplan C, and Blau J (2005) Circadian pacemaker neurons transmit and modulate visual information to control a rapid behavioral response. Neuron 45:293-300.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• McCabe C and Birley A (1998) Oviposition in the period genotypes of Drosophila melanogaster. Chronobiol Int 15:119-133.[Web of Science][Medline] [Order article via Infotrieve]
• Myers EM, Yu J, and Sehgal A (2003) Circadian control of eclosion: Interaction between a central and peripheral clock in Drosophila melanogaster. Curr Biol 13:526-533.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Paranjpe DA, Anitha D, Joshi A, and Sharma VK (2004) Multi-oscillatory control of eclosion and oviposition rhythms in Drosophila melanogaster: Evidence from limits of entrainment studies. Chronobiol Int 21:1-14.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Peng Y, Stoleru D, Levine JD, Hall JC, and Rosbash M (2003) Drosophila free-running rhythms require intercellular communication. PLOS Biol 1:1-9.
• Pittendrigh CS (1960) Circadian rhythms and the circadian organization of living systems. Cold Spring Harb Symp Quant Biol 25:159-182.[Abstract/Free Full Text]
• Renn SCP, Park JH, Rosbash M, Hall JC, and Taghert PH (1999) A pdf neuropeptide gene mutation and ablation of PDF neurons each cause severe abnormalities of behavioral circadian rhythms in Drosophila. Cell 99:791-802.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Saunders DS (1982) Insect Clocks. Oxford, UK: Pergamon.
• Sharma VK (2003) Simple computer aided device for monitoring activity of small mammals and insects. Biol Rhythm Res 34:3-12.
• Sheeba V, Chandrashekaran MK, Joshi A, and Sharma VK (2001a) Persistence of oviposition rhythm in individuals of Drosophila melanogaster reared in an aperiodic environment for several hundred generations. J Exp Zool 290:541-549.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Sheeba V, Chandrashekaran MK, Joshi A, and Sharma VK (2001b) A case for multiple oscillators controlling different circadian rhythms in Drosophila melanogaster. J Insect Physiol 47:1217-1225.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Siegel FG (1980) Testing for periodicity in a time series. Am Stat Assoc 75:345-348.[CrossRef]
• STATISTICATM (1995) Statistics II. Tulsa, OK: Statsoft.
• Steller H, Fischbach KF, and Rubin G (1987) A locus required for neuronal pathway function in the visual system of Drosophila. Cell 50:1139-1153.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Zerr DM, Hall JC, Rosbash M, and Siwicki KK (1990) Circadian fluctuations of period protein immunoreactivity in the CNS and the visual system of Drosophila. J Neurosci 10:2749-2762.[Abstract]

Journal of Biological Rhythms, Vol. 21, No. 1, 13-20 (2006)
DOI: 10.1177/0748730405282882


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Web of Science (4) Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Howlader, G. Articles by Sharma, V. K. Search for Related Content PubMed PubMed Citation Articles by Howlader, G. Articles by Sharma, V. K. Social Bookmarking                         What's this?