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Behavior in Light-Dark Cycles of Drosophila Mutants That Are Arrhythmic, Blind, or Both

Department of Biology, Brandeis University, Waltham, Massachusetts 02254

Melanie J. Hamblen-Coyle

Department of Biology, Brandeis University, Waltham, Massachusetts 02254

Mitchell S. Dushay

Department of Biology, Brandeis University, Waltham, Massachusetts 02254

Jeffrey C. Hall

Department of Biology, Brandeis University, Waltham, Massachusetts 02254

Certain of the rhythm mutations in Drosophila melanogaster lead to arrhythmic locomotor activity (and aperiodic eclosion) in constant conditions. In light-dark (LD) cycles, however, such mutants exhibit clear fluctuations between high levels of activity when the lights are on and much lower ones when they are off. Our data, in contrast to some previous conclusions, strongly suggest that period⁰ (per⁰) adults are, in LD conditions, merely being "forced" into exhibiting periodic behavior. These experiments involved application of 8-, 12-, 16-, and 24-hr LD cycles, in which the arrhythmic mutant could have any of these periodicities imposed upon it, whereas wild-type flies tended to exhibit periods of about 24 hr in cycling conditions whose T values were >8 hr different from 24. In phase-shift experiments, it was found that Drosophila expressing genotypes associated with rhythmicity achieved a 5-hr advance over a 2-day period following an advanced lights-on; per⁰ adults altered the phase of their locomotor peaks more rapidly. Against a background of the fact that eyeless or blind flies exhibit normal entrainment, it was hypothesized that double-mutant flies— carrying such visual mutations and per⁰ as well—should not synchronize to LD cycles, if the forced rhythms seen in the latter single-mutant type are mediated solely by light input through the external photoreceptors. Since an appreciable proportion of the double mutants did synchronize (to LD 12:12), it is thus suggested that the visual cues involved in forcing rhythmicity could be input through the same extraocular photoreceptors that, in general, subserve the fly's rhythm system.

Key Words: period0 • disconnected • sine oculis • and no-receptor-potential mutants • photoreceptors • locomotor activity • limits of entrainment • phase shifts

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