Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here for more information

Sign In to gain access to subscriptions and/or personal tools.
Journal of Biological Rhythms
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Lakin-Thomas, P. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lakin-Thomas, P. L.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Phase Resetting of the Neurospora crassa Circadian Oscillator: Effects of Inositol Depletion on Sensitivity to Light

Patricia L. Lakin-Thomas

Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, United Kingdom

The input pathway between the blue-light photoreceptor and the circadian oscillator of Neurospora crassa has not yet been identified. To test the hypothesis that an inositol phospholipid signaling system might be involved in blue-light signal transduction, phase resetting by light was assayed in the inositol-requiring inl strain under conditions of inositol depletion. Phase-resetting curves and dose-response curves indicated that cultures maintained on low inositol (25 µM) were several orders of magnitude more sensitive to light than those maintained on high inositol (250 µM). This difference in light sensitivity was a property of inositol auxotrophy and was not seen in the wild type or in an inositol-independent inl + revertant. Phase resetting by temperature was not affected by inositol depletion, indicating that the effect on light resetting is specific to the light input pathway and is not the result of a change in the amplitude of the oscillator itself. The results indicate an indirect role for inositol metabolites in the light input pathway—one that is not likely to involve direct participation of an inositol phospholipid signal transduction mechanism.

Key Words: Neurospora • circadian rhythm • clock • inositol • phase resetting • light • temperature

Journal of Biological Rhythms, Vol. 7, No. 3, 227-239 (1992)
DOI: 10.1177/074873049200700304
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Eukaryot CellHome page
R. R. Lew, Z. Abbas, M. I. Anderca, and S. J. Free
Phenotype of a Mechanosensitive Channel Mutant, mid-1, in a Filamentous Fungus, Neurospora crassa
Eukaryot. Cell, April 1, 2008; 7(4): 647 - 655.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. L. Lakin-Thomas and S. Brody
Circadian rhythms in Neurospora crassa: Lipid deficiencies restore robust rhythmicity to null frequency and white-collar mutants
PNAS, January 4, 2000; 97(1): 256 - 261.
[Abstract] [Full Text] [PDF]

Home page
 J Biol RhythmsHome page
P. L. Lakin-Thomas
Choline Depletion, frq Mutations, and Temperature Compensation of the Circadian Rhythm in Neurospora crassa
J Biol Rhythms, August 1, 1998; 13(4): 268 - 277.
[Abstract] [PDF]