| Sign In to gain access to subscriptions and/or personal tools. |
Circadian Variations in Clock Gene Expression of Human Bone Marrow CD34+ CellsStem Cell Research Group, the Gade Institute, Department of Pathology, Haukeland University Hosptial, Bergen, Norway, ole.larum{at}gades.uib.no.
Department of Medicine, Haukeland University Hospital, Bergen, Norway
Center for Medical Genetics and Molecular Medicine, Haukeland University Hospital, Bergen, Norway
The Rhythmometry Laboratory, College of Biological Sciences, Biological Sciences Center, University of Minnesota, St. Paul
Department of Cardiology, Haukeland University Hospital, Bergen, Norway
Stem Cell Research Group, the Gade Institute, Department of Pathology, Haukeland University Hosptial, Bergen, Norway
Center for Medical Genetics and Molecular Medicine, Haukeland University Hospital, Bergen, Norway
Department of Medicine, Haukeland University Hospital, Bergen, Norway
Center for Medical Genetics and Molecular Medicine, Haukeland University Hospital, Bergen, Norway
Stem Cell Research Group, the Gade Institute, Department of Pathology, Haukeland University Hosptial, Bergen, Norway
Time-dependent variations in clock gene expression have recently been observed in mouse hematopoietic cells, but the activity of these genes in human bone marrow (BM) has so far not been investigated. Since such data can be of considerable clinical interest for monitoring the dynamics in stem/progenitor cells, the authors have studied mRNA expression of the clock genes hPer1 , hPer2, hCry1, hCry2, hBmal1, hRev-erb
Key Words: hematopoiesis • clock genes • stem cells • human bone marrow • circadian rhythm
Journal of Biological Rhythms, Vol. 22, No. 2,
140-150 (2007) This article has been cited by other articles:
|
|||||||||||||||
 |
|
|
 |
|
Sign In to gain access to subscriptions and/or personal tools.
, and hClock in human hematopoietic CD34-positive (CD34 +) cells. CD34+ cells were isolated from the BM samples obtained from 10 healthy men at 6 times over 24 h. In addition, clock gene mRNA expression was analyzed in the whole BM in 3 subjects. Rhythms in serum cortisol, growth hormone, testosterone, and leukocyte counts documented that subjects exhibited standardized circadian patterns. All 7 clock genes were expressed both in CD34+ cells and the whole BM, with some differences in magnitude between the 2 cell populations. A clear circadian rhythm was shown for hPer1, hPer2, and hCry2 expression in CD34+ cells and for hPer1 in the whole BM, with maxima from early morning to midday. Similar to mouse hematopoietic cells, h Bmal1 was not oscillating rhythmically. The study demonstrates that clock gene expression in human BM stem/progenitor cells may be developmentally regulated, with strong or weaker circadian profiles as compared to those reported in other mature tissues. 
