This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by de Paula, R. M. Articles by Bell-Pedersen, D. Search for Related Content PubMed PubMed Citation Articles by de Paula, R. M. Articles by Bell-Pedersen, D. Pubmed/NCBI databases Gene Social Bookmarking                         What's this?

Two Circadian Timing Circuits in Neurospora crassa Cells Share Components and Regulate Distinct Rhythmic Processes

Center for Research on Biological Clocks, Department of Biology, Texas A&M University, College Station, TX

Zachary A. Lewis

Center for Research on Biological Clocks, Department of Biology, Texas A&M University, College Station, TX

Andrew V. Greene

Center for Research on Biological Clocks, Department of Biology, Texas A&M University, College Station, TX

Kyung Suk Seo

Center for Research on Biological Clocks, Department of Biology, Texas A&M University, College Station, TX

Louis W. Morgan

Center for Research on Biological Clocks, Department of Biology, Texas A&M University, College Station, TX

Michael W. Vitalini

Center for Research on Biological Clocks, Department of Biology, Texas A&M University, College Station, TX

Lindsay Bennett

Center for Research on Biological Clocks, Department of Biology, Texas A&M University, College Station, TX

Richard H. Gomer

Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005

Deborah Bell-Pedersen

Center for Research on Biological Clocks, Department of Biology, Texas A&M University, College Station, TX, dpedersen{at}mail.bio.tamu.edu

In Neurospora crassa, FRQ, WC-1, and WC-2 proteins comprise the core circadian FRQ-based oscillator that is directly responsive to light and drives daily rhythms in spore development and gene expression. However, physiological and biochemical studies have demonstrated the existence of additional oscillators in the cell that function in the absence of FRQ (collectively termed FRQ-less oscillators [FLOs]). Whether or not these represent temperature-compensated, entrainable circadian oscillators is not known. The authors previously identified an evening-peaking gene, W06H2 (now called clock-controlled gene 16 [ccg-16]), which is expressed with a robust daily rhythm in cells that lack FRQ protein, suggesting that ccg-16 is regulated by a FLO. In this study, the authors provide evidence that the FLO driving ccg-16 rhythmicity is a circadian oscillator. They find that ccg-16 rhythms are generated by a temperature-responsive, temperature-compensated circadian FLO that, similar to the FRQ-based oscillator, requires functional WC-1 and WC-2 proteins for activity. They also find that FRQ is not essential for rhythmic WC-1 protein levels, raising the possibility that this WCFLO is involved in the generation of WC-1 rhythms. The results are consistent with the presence of 2 circadian oscillators within Neurospora cells, which the authors speculate may interact with each other through the shared WC proteins.

Key Words: circadian • oscillator • Neurospora • FREQUENCY • WC-1 • FRQ-less oscillator • ccg-16 • WC-FLO

Journal of Biological Rhythms, Vol. 21, No. 3, 159-168 (2006)
DOI: 10.1177/0748730406288338


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				K. Schneider, S. Perrino, K. Oelhafen, S. Li, A. Zatsepin, P. Lakin-Thomas, and S. Brody Rhythmic Conidiation in Constant Light in Vivid Mutants of Neurospora crassa Genetics, March 1, 2009; 181(3): 917 - 931. [Abstract] [Full Text] [PDF]

				M. Shi, L. F. Larrondo, J. J. Loros, and J. C. Dunlap A developmental cycle masks output from the circadian oscillator under conditions of choline deficiency in Neurospora PNAS, December 11, 2007; 104(50): 20102 - 20107. [Abstract] [Full Text] [PDF]

				X. Xu, C. T. Hotta, A. N. Dodd, J. Love, R. Sharrock, Y. W. Lee, Q. Xie, C. H. Johnson, and A. A.R. Webb Distinct Light and Clock Modulation of Cytosolic Free Ca2+ Oscillations and Rhythmic CHLOROPHYLL A/B BINDING PROTEIN2 Promoter Activity in Arabidopsis PLANT CELL, November 1, 2007; 19(11): 3474 - 3490. [Abstract] [Full Text] [PDF]

				S. M. Hunt, M. Elvin, S. K. Crosthwaite, and C. Heintzen The PAS/LOV protein VIVID controls temperature compensation of circadian clock phase and development in Neurospora crassa Genes & Dev., August 1, 2007; 21(15): 1964 - 1974. [Abstract] [Full Text] [PDF]

				H. G. McWatters, E. Kolmos, A. Hall, M. R. Doyle, R. M. Amasino, P. Gyula, F. Nagy, A. J. Millar, and S. J. Davis ELF4 Is Required for Oscillatory Properties of the Circadian Clock Plant Physiology, May 1, 2007; 144(1): 391 - 401. [Abstract] [Full Text] [PDF]

				L. Lombardi, K. Schneider, M. Tsukamoto, and S. Brody Circadian Rhythms in Neurospora crassa: Clock Mutant Effects in the Absence of a frq-Based Oscillator Genetics, March 1, 2007; 175(3): 1175 - 1183. [Abstract] [Full Text] [PDF]

				J.C. Dunlap, J.J. Loros, H.V. Colot, A. Mehra, W.J. Belden, M. Shi, C.I. Hong, L.F. Larrondo, C.L. Baker, C.-H. Chen, et al. A Circadian Clock in Neurospora: How Genes and Proteins Cooperate to Produce a Sustained, Entrainable, and Compensated Biological Oscillator with a Period of about a Day Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 57 - 68. [Abstract] [PDF]

				J. J. Loros, J. C. Dunlap, L. F. Larrondo, M. Shi, W. J. Belden, V. D. Gooch, C.-H. Chen, C. L. Baker, A. Mehra, H. V. Colot, et al. Circadian Output, Input, and Intracellular Oscillators: Insights into the Circadian Systems of Single Cells Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 201 - 214. [Abstract] [PDF]

				M. Merrow and T. Roenneberg Circadian Entrainment of Neurospora crassa Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 279 - 285. [Abstract] [PDF]

				R. M. de Paula, M. W. Vitalini, R. H. Gomer, and D. Bell-Pedersen Complexity of the Neurospora crassa Circadian Clock System: Multiple Loops and Oscillators Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 345 - 351. [Abstract] [PDF]

				M. W. Vitalini, R. M. de Paula, W. D. Park, and D. Bell-Pedersen The Rhythms of Life: Circadian Output Pathways in Neurospora J Biol Rhythms, December 1, 2006; 21(6): 432 - 444. [Abstract] [PDF]

				J. C. Dunlap Proteins in the Neurospora Circadian Clockworks J. Biol. Chem., September 29, 2006; 281(39): 28489 - 28493. [Full Text] [PDF]

				Y. Liu and D. Bell-Pedersen Circadian Rhythms in Neurospora crassa and Other Filamentous Fungi. Eukaryot. Cell, August 1, 2006; 5(8): 1184 - 1193. [Full Text] [PDF]