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Are Protein Synthesis Inhibition and Phase Shifting of the Circadian Clock in Gonyaulax Correlated?

Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138

Anne Ungar

Department of Biochemistry, Molecular and Cell Biology, Northwestern University, Evanston, Illinois 60201.

Carl Hirschie Johnson

Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138

J. Woodland Hastings

Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138

We describe a method whereby the effect of protein synthesis inhibitors upon protein synthesis in Gonyaulax cultures may be reliably measured. Using this method, we found that protein synthesis inhibition and clock resetting were correlated, but that the correlation was not as close as has been reported in other systems. The effect of the inhibitors anisomycin and cycloheximide upon phase shifting of the circadian clock was a function of the illumination and temperature conditions to which the cells were subjected, but these factors did not appear to influence the inhibition of protein synthesis by these drugs. Cellular protein synthesis did not recover immediately from the inhibitors' effects; depending upon the previous concentration of the inhibitor, translational recovery from the drugs may require hours. This observation has important implications for the analysis of any phase response curve when the stimulus is a chemical.

Journal of Biological Rhythms, Vol. 2, No. 2, 121-138 (1987)
DOI: 10.1177/074873048700200204


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