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Testicular Apoptosis Is Down-Regulated during Spontaneous Recrudescence in White-Footed Mice (Peromyscus leucopus)Division of Reproductive Sciences, OregonRegional Primate Research Center, Oregon Health Sciences University, 505 NW 185th Avenue, Beaverton, OR 97006, USA
Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Johns Hopkins School of Public Health, Baltimore, MD 21216, USA
Departments of Psychology and Neuroscience, The Ohio State University, Columbus, OH 43210, USA; The Ohio State University, Department of Psychology, 009 Townshend Hall, 1885 Neil Avenue Mall, Columbus, OH 43210-1222, USA; rnelson{at}osu.edu Among individuals of many nontropical species, seasonal breeding is timed by tracking changes in the daily photoperiod. Transfer of rodents to short (< 12 h of light/day) day lengths for 6 to 14 weeks can induce regression of the testes mediated by apoptosis. After 16 to 20 weeks of short day exposure, reproductive function is "spontaneously" initiated, and testicular recrudescence is observed. The gonadal mechanisms that underlie testicular recrudescence are not fully understood. If the onset of testicular regrowth that occursduring spontaneous recrudescence reflects a down-regulation of apoptotic signals, then a decline in apoptosis should be noted concurrent with increased testis mass. This experiment sought toassess the role of apoptosis in the restoration of reproductive capacity to photoperiod-inhibited white-footed mice. Males were assigned to long (16:8 LD) or short (8:16 LD) photoperiods for0, 14, 18, 22, 26, or 30 weeks. At each of these time points, testis mass and testosterone concentrations were assessed. In addition, apoptotic activity was measured using both in situ terminal deoxynucleotidyl transferase dNTP end labeling (TUNEL) and DNAladdering. Short photoperiod exposure induced maximal decreases in testicular parameters after 14 weeks (p <0.05). After 26 weeks of short days, testis mass was no longer different between males housed in long days and those housed in short days. In contrast, the high incidence of apoptotic TUNEL labeling and DNAladdering observed at 14 weeks was reduced to long day values after 22 weeks ofshort day exposure. Together, our results establish that a decrease in testicular apoptosis coincides with testicular recrudescence in white-footed mice. The current study demonstrates a decline in the incidence of testicular cell death concomitant with changes in testis mass or length, elucidating a timeline of changes at the cellular level related to the onset of recrudescence.
Key Words: recrudescence • apoptosis • photoperiod • TUNEL • seasonal reproduction • testis • Peromyscus
Journal of Biological Rhythms, Vol. 16, No. 5,
479-488 (2001) This article has been cited by other articles:
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