|
Sign In to gain access to subscriptions and/or personal tools.
|
Specific Sequences Outside the E-box Are Required for Proper per Expression and Behavioral Rescue
Lisa C. Lyons
Department of Biology and Biochemistry, University of Houston, Houston, TX 772040-5513, USA
Thomas K. Darlington
Department of Cell Biology and NSF Center for Biological Timing, The Scripps Research Institute, 10550 North Torrey Pines Road, LaJolla, CA 92037, USA
Haiping Hao
Rohm Haas Research Laboratories, 727 Norristown Road, Spring House, PA 19477, USA
Jerry Houl
Department of Biology and Biochemistry, University of Houston, Houston, TX 772040-5513, USA
Steve A. Kay
Department of Cell Biology and NSF Center for Biological Timing, The Scripps Research Institute, 10550 North Torrey Pines Road, LaJolla, CA 92037, USA
Paul E. Hardin
Department of Biology and Biochemistry, University of Houston, Houston, TX 772040-5513, USA
A 69 bp circadian regulatory sequence (CRS) upstream of the per gene is sufficient to drive circadian transcription, mediate proper spatial expression, and rescue behavioral rhythmicity in per01 flies. Within the CRS, an E-box is required for transcriptional activation by two basic-helix-loop-helix (bHLH) PERARNT-SIM (PAS) transcription factors, dCLOCK (dCLK) and CYCLE (CYC). To define sequences within the CRS that are required for spatial expression, circadian expression, and behavioral rhythmicity, a series of mutants that alter blocks of 3 to 12 nucleotides across the entire CRS were used to drive lacZ or per expression in vivo. As expected, the E-box within the CRS is necessary for high-level expression and behavioral rhythmicity, but sequences outside the E-box are also required for transcriptional activation, proper spatial expression, and behavioral rhythmicity. These results indicate that the dCLK-CYC target site extends beyond the E-box and that factors other than dCLK and CYC modulate per transcription.
Key Words: Drosophila • period • circadian rhythms • transcription • gene expression • behavior
Journal of Biological Rhythms, Vol. 15, No. 6,
472-482 (2000)
DOI: 10.1177/074873040001500604
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
J.M. Fustin, J.S. O'Neill, M.H. Hastings, D.G. Hazlerigg, and H. Dardente
Cry1 Circadian Phase in vitro: Wrapped Up with an E-Box
J Biol Rhythms,
February 1, 2009;
24(1):
16 - 24.
[Abstract]
[PDF]
|
|
|
|
|
|
|
P. Taylor and P. E. Hardin
Rhythmic E-Box Binding by CLK-CYC Controls Daily Cycles in per and tim Transcription and Chromatin Modifications
Mol. Cell. Biol.,
July 15, 2008;
28(14):
4642 - 4652.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Andronis, S. Barak, S. M. Knowles, S. Sugano, and E. M. Tobin
The Clock Protein CCA1 and the bZIP Transcription Factor HY5 Physically Interact to Regulate Gene Expression in Arabidopsis
Mol Plant,
January 1, 2008;
1(1):
58 - 67.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Hyuk Wan Ko, S. DiMassa, Eun Young Kim, K. Bae, and I. Edery
Cis-Combination of the Classic perS and perL Mutations Results in Arrhythmic Drosophila with Ectopic Accumulation of Hyperphosphorylated PERIOD Protein
J Biol Rhythms,
December 1, 2007;
22(6):
488 - 501.
[Abstract]
[PDF]
|
|
|
|
|
|
|
A. Matsumoto, M. Ukai-Tadenuma, R. G. Yamada, J. Houl, K. D. Uno, T. Kasukawa, B. Dauwalder, T. Q. Itoh, K. Takahashi, R. Ueda, et al.
A functional genomics strategy reveals clockwork orange as a transcriptional regulator in the Drosophila circadian clock
Genes & Dev.,
July 1, 2007;
21(13):
1687 - 1700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-F. Chen, J. Majercak, and I. Edery
Clock-Gated Photic Stimulation of Timeless Expression at Cold Temperatures and Seasonal Adaptation in Drosophila
J Biol Rhythms,
August 1, 2006;
21(4):
256 - 271.
[Abstract]
[PDF]
|
|
|
|
|
|
|
W. Yu, H. Zheng, J. H. Houl, B. Dauwalder, and P. E. Hardin
PER-dependent rhythms in CLK phosphorylation and E-box binding regulate circadian transcription.
Genes & Dev.,
March 15, 2006;
20(6):
723 - 733.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Harmer and S. A. Kay
Positive and Negative Factors Confer Phase-Specific Circadian Regulation of Transcription in Arabidopsis
PLANT CELL,
July 1, 2005;
17(7):
1926 - 1940.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Preuss, J.-Y. Fan, M. Kalive, S. Bao, E. Schuenemann, E. S. Bjes, and J. L. Price
Drosophila doubletime Mutations Which either Shorten or Lengthen the Period of Circadian Rhythms Decrease the Protein Kinase Activity of Casein Kinase I
Mol. Cell. Biol.,
January 15, 2004;
24(2):
886 - 898.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Froehlich, J. J. Loros, and J. C. Dunlap
Rhythmic binding of a WHITE COLLAR-containing complex to the frequency promoter is inhibited by FREQUENCY
PNAS,
May 13, 2003;
100(10):
5914 - 5919.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Liu and C. B. Green
Circadian Regulation of nocturnin Transcription by Phosphorylated CREB in Xenopus Retinal Photoreceptor Cells
Mol. Cell. Biol.,
November 1, 2002;
22(21):
7501 - 7511.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. P. Michael and C. R. McClung
Phase-Specific Circadian Clock Regulatory Elements in Arabidopsis
Plant Physiology,
October 1, 2002;
130(2):
627 - 638.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Munoz, M. Brewer, and R. Baler
Circadian Transcription. THINKING OUTSIDE THE E-BOX
J. Biol. Chem.,
September 20, 2002;
277(39):
36009 - 36017.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P. Kyriacou and E. Rosato
Squaring Up the E-box
J Biol Rhythms,
December 1, 2000;
15(6):
483 - 490.
[PDF]
|
|
|
|
|
