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Probing the Circadian Pacemaker of a Mouse Using Two Light Pulses

Chronobiology Laboratory, Evolutionary and Organismal Biology Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, P.O. Box. 6436, Bangalore-560 064, Karnataka, India

R. Chidambaram

Department of Animal Behaviour and Physiology, School of Biological Sciences, Madurai Kamaraj University, Madurai-625 021, Tamil Nadu, India

Maroli K. Chandrashekaran

Chronobiology Laboratory, Evolutionary and Organismal Biology Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, P.O. Box. 6436, Bangalore-560 064, Karnataka, India

In two separate sets of experiments, the phases of the locomotor activity rhythm of the nocturnal field mouse Mus booduga were probed using two light pulses (LPs). In the first set of experiments, the circadian pacemaker underlying the locomotor activity rhythm was perturbed at circadian time 14 (CT 14) using a resetting light pulse LP₁ of 1000 lux intensity and 15 min duration. The phases of the resetting pacemaker were then probed at all even CTs between CT 16 and CT 14 using a PRC probing light pulse LP₂ of equal strength. The "LP₂PRC" thus obtained was then compared with the single light pulse PRC in terms of the area under delay (D) and advance (A) zones of the PRCs. The time course and waveform of the two LP PRCs suggest that the LP₂PRC resembled the single LP PRC, displaced by 2 h toward the right. The LP₂PRC had smaller D compared to the single LPPRC (p = 0.007), whereas both the PRCs had A of equal magnitude (p = 0.23). This suggests that the pacemaker phase shifts rapidly after LP perturbations. In the second set of experiments, the LP₁ was administered at CT 14. The phase of the pacemaker was then perturbed on day 1 (next cycle after LP₁) either 2 h after activity onset (at ca. CT 14 of the transient cycle) or 8 h after activity onset (at ca. CT 20 of the transient cycle) using an LP₂ of equal strength. It was observed that the steady-state phase shifts evoked by positioning an LP₂, 2 h after activity onset, were positively correlated with the phase shifts observed on day 1. The steady-state phase shifts observed, when the LP₂ was positioned, 8 h after activity onset, were negatively correlated with the phase shifts observed on day 1. These results suggest that the transient cycles do not mirror the state of the pacemaker oscillator.

Key Words: circadian pacemaker • phase response curve • transients • E and M oscillators

Journal of Biological Rhythms, Vol. 15, No. 1, 67-73 (2000)
DOI: 10.1177/074873040001500108


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