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Temporal and Spatial Expression of the period Gene in the Reproductive System of the Codling Moth

Department of Entomology, Oregon State University, Corvallis, OR 97331-2907, USA

Jason A. Kilgore

Department of Entomology, Oregon State University, Corvallis, OR 97331-2907, USA; Molecular Probes, Inc., 4849 Pitchford Ave., Eugene, 97402-9165.

Piotr Bebas

Warsaw University, Department of Invertebrate Physiology, Zoological Institute, 93 Zwirki & Wigury St., Warsaw, Poland 02-089

Jadwiga M. Giebultowicz

Department of Entomology, Oregon State University, Corvallis, OR 97331-2907, USA

The authors examined patterns of spatial and temporal expression of Drosophila per gene homologue in the codling moth, Cydia pomonella. Since sperm release in moths is regulated in a circadian manner by an autonomous clock that is independent from the brain, the authors investigated per expression in male reproductive system along with its expression in moth heads. per mRNA is rhythmically expressed with the same phase and amplitude in both tissues under light-dark (LD) conditions. The levels of per mRNA are low during the day, start to increase before lights-off, reach the peak in dark, and decrease after lights-on. In constant darkness (DD), cycling of per mRNA continued in heads with severely blunted amplitude. No cycling of per mRNA was detected in testis in DD. In situ hybridization and immunocytochemistry revealed distinct spatial patterns of per expression in the moth reproductive system. There is no expression of per in cells forming the wall of testes or in sperm bundles. However, permRNA and protein are rhythmically expressed in the epithelial cells forming the wall of the upper vas deferens (UVD) and in the cells of the terminal epithelium, which are involved in the circadian gating of sperm release. Increase in permRNA in the UVD coincides with sperm accumulation in this part of the insect reproductive system.

Key Words: per • circadian rhythms • reproduction • insects

Journal of Biological Rhythms, Vol. 15, No. 1, 4-12 (2000)
DOI: 10.1177/074873040001500102


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