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A Reexamination of the Role of GABA in the Mammalian Suprachiasmatic Nucleus

Rick L. Pieschl

Todd A. Wisialowski

Neuroscience Electrophysiology, Department 409, Bristol-Myers Squibb Pharmaceutical Research Institute, 5 Research Parkway, Wallingford, CT 06492

Won K. Park

George J. Strecker

Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, CO 80523

Marcel T. G. de Jeu

Cyriel M. A. Pennartz

Netherlands Institute for Brain Research, Meibergdreef 33, 1105 AZ, Amsterdam, The Netherlands

F. Edward Dudek

Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, CO 80523

Three independent electrophysiological approaches in hypothalamic slices were used to test the hypothesis that [.gamma]-amino butyric acid (GABA)_Areceptor activation excites suprachiasmatic nucleus (SCN) neurons during the subjective day, consistent with a recent report. First, multiple-unit recordings during either the subjective day or night showed that GABA or muscimol inhibited firing activity of the SCN population in a dose-dependent manner. Second, cell-attached recordings during the subjective day demonstrated an inhibitory effect of bath-or microapplied GABA on action currents of single SCN neurons. Third, gramicidin perforated-patch recordings showed that bicuculline increased the spontaneous firing rate during the subjective day. Therefore, electrophysiological data obtained by three different experimental methods provide evidence that GABA is inhibitory rather than excitatory during the subjective day.

Key Words: circadian • GABAA • hypothalamus • muscimol • bicuculline

Journal of Biological Rhythms, Vol. 14, No. 2, 126-130 (1999)
DOI: 10.1177/074873099129000515


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