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Journal of Biological Rhythms
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Circadian Rhythmicity of Vasopressin Levels in the Cerebrospinal Fluid of Suprachiasmatic Nucleus-Lesioned and Grafted Rats

K. E. Lisette van Esseveldt

Graduate School Neurosciences Amsterdam, Netherlands Institute for Brain Research, Meibergdreef 33, 1105 AZ Amsterdam ZO, The Netherlands

Ben A. M. van der Geest

Hans Duindam

Wop J. Rietveld

Department of Physiology and Physiological Physics, Division of Chronobiology, University of Leiden, P.O. Box 9604, 2300 RC Leiden, The Netherlands

Gerard J. Boer

Graduate School Neurosciences Amsterdam, Netherlands Institute for Brain Research, Meibergdreef 33, 1105 AZ Amsterdam ZO, The Netherlands

Transplantation of the fetal suprachiasmatic nucleus (SCN) in arrhythmic SCN-lesioned rats can reinstate circadian drinking rhythms in 40% to 50% of the cases. In the current article, it was investigated whether the failure in the other rats could be due to the absence of a circadian rhythm in the grafted SCN, using a circadian vasopressin (VP) rhythm in the cerebrospinal fluid (CSF) as the indicator for a rhythmic SCN. CSF was sampled in continuous darkness from intact control rats and SCN-lesioned and grafted rats. VP could be detected in all samples, with concentrations of 15 to 30 pg/ml in the control rats and 5 to 15 pg/ml in the grafted rats. A circadian VP rhythm with a two-to threefold difference between peak and nadir values was found in all 7 control rats but in only 4 of 13 experimental rats, despite the presence of a VP-positive SCN in all grafts. A circadian VP rhythm was present in 2 drinking rhythm-recovered rats (6 of 13) and in 2 nonrecovery rats. Apparently, in these latter rats, the failure of the grafted SCN to restore a circadian drinking rhythm cannot be attributed to a lack of rhythmicity in the SCN itself. Thus, the presence of a rhythmic grafted SCN, as is deduced from a circadian CSF VP rhythm, appears not to be sufficient for restoration of a circadian drinking rhythm in SCN-lesioned arrhythmic rats.

Key Words: vasopressin • suprachiasmatic nucleus • neurotransplantation • cerebrospinal fluid • circadian rhythm

Journal of Biological Rhythms, Vol. 14, No. 1, 28-36 (1999)
DOI: 10.1177/074873099129000416
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