This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Rutila, J. E. Articles by Rosbash, M. Search for Related Content PubMed PubMed Citation Articles by Rutila, J. E. Articles by Rosbash, M. Social Bookmarking                         What's this?

The tim^SL Mutant Affects a Restricted Portion of the Drosophila melanogaster Circadian Cycle

Howard Hughes Medical Institute, Brandeis University, Department of Biology, Waltham, MA 02454-9110

Olga Maltseva

Department of Ophthalmology, Mount Sinai Medical Center, New York, NY 10029

Michael Rosbash

Howard Hughes Medical Institute, Brandeis University, Department of Biology, Waltham, MA 02454-9110

The circadian rhythm genes period (per) and timeless (tim) are central to contemporary studies on Drosophila circadian rhythms. Mutations in these genes give rise to arrhythmic or period-altered phenotypes, and per and tim gene expression is under clock control. per and tim proteins (PER and TIM) also undergo circadian changes in level and phosphorylation state. The authors previously described a period-altering tim mutation, timSL, with allele-specific effects in different per backgrounds. This mutation also affected the TIM phosphorylation profile during the mid-late night. The authors show here that the single amino acid alteration in TIM-SL is indeed responsible for the phenotype, as a timSL transgene recapitulates the original mutant phenotype and shortens the period of perL flies by 3 h. The authors also show that this mutation has comparable effects in a light-dark cycle, as timSL also accelerates the activity offset during the mid-late night of perL flies. Importantly, timSL advances predominantly the mid-late night region of the perL phase response curve, consistent with the notion that this portion of the cycle is governed by unique rate-limiting steps. The authors propose that TIM and PER phosphorylation are normally rate determining during the mid-late night region of the circadian cycle.

Key Words: Drosophila • circadian rhythms • behavior • timeless

Journal of Biological Rhythms, Vol. 13, No. 5, 380-392 (1998)
DOI: 10.1177/074873098129000200


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				S. Kadener, D. Stoleru, M. McDonald, P. Nawathean, and M. Rosbash Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component Genes & Dev., July 1, 2007; 21(13): 1675 - 1686. [Abstract] [Full Text] [PDF]

				N. Peschel, S. Veleri, and R. Stanewsky Veela defines a molecular link between Cryptochrome and Timeless in the light-input pathway to Drosophila's circadian clock PNAS, November 14, 2006; 103(46): 17313 - 17318. [Abstract] [Full Text] [PDF]

				S. A. Cyran, G. Yiannoulos, A. M. Buchsbaum, L. Saez, M. W. Young, and J. Blau The Double-Time Protein Kinase Regulates the Subcellular Localization of the Drosophila Clock Protein Period J. Neurosci., June 1, 2005; 25(22): 5430 - 5437. [Abstract] [Full Text] [PDF]

				Y. B. Kiyohara, M. Katayama, and T. Kondo A Novel Mutation in kaiC Affects Resetting of the Cyanobacterial Circadian Clock J. Bacteriol., April 15, 2005; 187(8): 2559 - 2564. [Abstract] [Full Text] [PDF]

				C. Wulbeck, G. Szabo, O. T. Shafer, C. Helfrich-Forster, and R. Stanewsky The Novel Drosophila timblind Mutation Affects Behavioral Rhythms but Not Periodic Eclosion Genetics, February 1, 2005; 169(2): 751 - 766. [Abstract] [Full Text] [PDF]

				L. M. Beaver, B. L. Rush, B. O. Gvakharia, and J. M. Giebultowicz Noncircadian Regulation and Function of Clock Genes Period and Timeless in Oogenesis of Drosophila Melanogaster J Biol Rhythms, December 1, 2003; 18(6): 463 - 472. [Abstract] [PDF]

				R. Stanewsky, K. S. Lynch, C. Brandes, and J. C. Hall Mapping of Elements Involved in Regulating Normal Temporal period and timeless RNA Expression Patterns in Drosophila melanogaster J Biol Rhythms, August 1, 2002; 17(4): 293 - 306. [Abstract] [PDF]

				L. M. Beaver, B. O. Gvakharia, T. S. Vollintine, D. M. Hege, R. Stanewsky, and J. M. Giebultowicz Loss of circadian clock function decreases reproductive fitness in males of Drosophilamelanogaster PNAS, February 19, 2002; 99(4): 2134 - 2139. [Abstract] [Full Text] [PDF]

				T. Stempfl, M. Vogel, G. Szabo, C. Wulbeck, J. Liu, J. C. Hall, and R. Stanewsky Identification of Circadian-Clock-Regulated Enhancers and Genes of Drosophila melanogaster by Transposon Mobilization and Luciferase Reporting of Cyclical Gene Expression Genetics, February 1, 2002; 160(2): 571 - 593. [Abstract] [Full Text] [PDF]

				M. J. McDonald, M. Rosbash, and P. Emery Wild-Type Circadian Rhythmicity Is Dependent on Closely Spaced E Boxes in the Drosophila timeless Promoter Mol. Cell. Biol., February 15, 2001; 21(4): 1207 - 1217. [Abstract] [Full Text] [PDF]

				V. Suri, J. C. Hall, and M. Rosbash Two Novel doubletime Mutants Alter Circadian Properties and Eliminate the Delay between RNA and Protein in Drosophila J. Neurosci., October 15, 2000; 20(20): 7547 - 7555. [Abstract] [Full Text] [PDF]