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Light-Induced Phase Shifting of the Circadian Conidiation Rhythm Is Inhibited by Calmodulin Antagonists in Neurospora crassa

Department of Biology, Faculty of Science, Okayama University, Tsushimanaka 3-1, Okayama 700, Japan

Hideaki Nakashima

Department of Biology, Faculty of Science, Okayama University, Tsushimanaka 3-1, Okayama 700, Japan

The effects of calmodulin antagonists and inhibitors of protein kinases and phosphatases on light-induced phase shifting were investigated in Neurospora crassa. Calmodulin antagonists, namely, trifluoperazine, chlorpromazine, and W-7, almost completely inhibited the light-induced phase shifting without having any effect on the circadian clock itself. Chlorpromazine was less effective in inhibiting the light-induced phase shifting than trifluoperazine. W-5, a dechlorinated analogue of W-7, failed to inhibit the light-induced phase shifting at the same concentration as that at which W-7 was effective. These results suggest that calmodulin is required during signal transduction from the light-perceiving system to the circadian clock in N. crassa. Inhibitors of protein phosphorylation did not inhibit the light-induced phase shifting, although these inhibitors completely inhibited mycelial growth. Trifluoperazine partially inhibited the phosphorylation of three proteins when phosphorylation was assayed in vitro.

Key Words: blue light • calmodulin antagonist • circadian clock • FK506 • Neurospora crassa • phase shifting • protein phosphorylation

Journal of Biological Rhythms, Vol. 11, No. 3, 234-240 (1996)
DOI: 10.1177/074873049601100305


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