This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Sadakane, Y. Articles by Nakashima, H. Search for Related Content PubMed PubMed Citation Articles by Sadakane, Y. Articles by Nakashima, H. Social Bookmarking                   What's this?

Light-Induced Phase Shifting of the Circadian Conidiation Rhythm Is Inhibited by Calmodulin Antagonists in Neurospora crassa

Department of Biology, Faculty of Science, Okayama University, Tsushimanaka 3-1, Okayama 700, Japan

Hideaki Nakashima

Department of Biology, Faculty of Science, Okayama University, Tsushimanaka 3-1, Okayama 700, Japan

The effects of calmodulin antagonists and inhibitors of protein kinases and phosphatases on light-induced phase shifting were investigated in Neurospora crassa. Calmodulin antagonists, namely, trifluoperazine, chlorpromazine, and W-7, almost completely inhibited the light-induced phase shifting without having any effect on the circadian clock itself. Chlorpromazine was less effective in inhibiting the light-induced phase shifting than trifluoperazine. W-5, a dechlorinated analogue of W-7, failed to inhibit the light-induced phase shifting at the same concentration as that at which W-7 was effective. These results suggest that calmodulin is required during signal transduction from the light-perceiving system to the circadian clock in N. crassa. Inhibitors of protein phosphorylation did not inhibit the light-induced phase shifting, although these inhibitors completely inhibited mycelial growth. Trifluoperazine partially inhibited the phosphorylation of three proteins when phosphorylation was assayed in vitro.

Key Words: blue light • calmodulin antagonist • circadian clock • FK506 • Neurospora crassa • phase shifting • protein phosphorylation

Journal of Biological Rhythms, Vol. 11, No. 3, 234-240 (1996)
DOI: 10.1177/074873049601100305


CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Katagiri, K. Onai, and H. Nakashima Spermidine Determines the Sensitivity to the Calmodulin Antagonist, Chlorpromazine, for the Circadian Conidiation Rhythm but Not for the Mycelial Growth in Neurospora crassa J Biol Rhythms, December 1, 1998; 13(6): 452 - 460. [Abstract] [PDF]