Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here for more information

Sign In to gain access to subscriptions and/or personal tools.
Journal of Biological Rhythms
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Gao, B.
Right arrow Articles by Moore, R. Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gao, B.
Right arrow Articles by Moore, R. Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Glutamic Acid Decarboxylase Message Isoforms in Human Suprachiasmatic Nucleus

Bo Gao

Departments of Psychiatry, Neurology, and Neuroscience, Center for Neuroscience, Alzheimer's Disease Research Center, and Center for the Neural Basis of Cognition, University of Pittsburgh, Pittsburgh, PA 15261

Robert Y. Moore

Departments of Psychiatry, Neurology, and Neuroscience, Center for Neuroscience, Alzheimer's Disease Research Center, and Center for the Neural Basis of Cognition, University of Pittsburgh, Pittsburgh, PA 15261

The rat suprachiasmatic nucleus (SCN) is comprised of neurons that contain gamma-amino butyric acid (GABA) colocalized with one or more peptides. In the present study, the authors employed in situ hybridization histochemistry to determine whether the human SCN also contains GABA neurons using synthetic oligonucleotide probes complementary to sequences of two isoforms of the GABA-forming enzyme, glutamic acid decarboxylase (GAD), GAD 65, and GAD67. Most, if not all, SCN neurons appear to express both GAD65 mRNA and GAD67 mRNA with the content of GAD67 greater than GAD65. Both isoforms also are expressed in some neurons of the anterior hypothalamic area, in small neurons of the paraventricular nucleus but not in the supraoptic nucleus. These data indicate that neurons in the human SCN, like those in rodents, use GABA as a neurotransmitter.

Key Words: GABA • GAD65 • GAD67 • in situ hybridization • circadian system • paraventricular nucleus • Alzheimer's disease

Journal of Biological Rhythms, Vol. 11, No. 2, 172-179 (1996)
DOI: 10.1177/074873049601100209
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Physiol.Home page
S. Wagner, N. Sagiv, and Y. Yarom
GABA-induced current and circadian regulation of chloride in neurones of the rat suprachiasmatic nucleus
J. Physiol., December 15, 2001; 537(3): 853 - 869.
[Abstract] [Full Text] [PDF]

Home page
 J Biol RhythmsHome page
T. A. Wehr
Photoperiodism in Humans and Other Primates: Evidence and Implications
J Biol Rhythms, August 1, 2001; 16(4): 348 - 364.
[Abstract] [PDF]