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Acquisition of Circadian Bioluminescence Data in Gonyaulax and an Effect of the Measurement Procedure on the Period of the Rhythm

Department of Cellular and Developmental Biology, Harvard University, The Biological Laboratories, Cambridge, Massachusetts 02138

Van D. Gooch

Department of Cellular and Developmental Biology, Harvard University, The Biological Laboratories, Cambridge, Massachusetts 02138

Walter Taylor

Department of Cellular and Developmental Biology, Harvard University, The Biological Laboratories, Cambridge, Massachusetts 02138

Nick Aiuto

Department of Cellular and Developmental Biology, Harvard University, The Biological Laboratories, Cambridge, Massachusetts 02138

J. Woodland Hastings

Department of Cellular and Developmental Biology, Harvard University, The Biological Laboratories, Cambridge, Massachusetts 02138

During measurements of the circadian (approximately 24-hr) rhythms of spontaneous bioluminescence in the marine dinoflagellate Gonyaulax polyedra, the individual cultures in vials were shielded from otherwise constant dim light for 1-3 min every 20-60 min by a photomultiplier housing that was moved from vial to vial. The high-frequency dark pulses caused a small but consistent shortening of the free-running circadian period, but there was no indication that the dark pulses caused entrainment. Hardware and software components of the microcomputer-controlled data collection system are described. A microcomputer controlled the movement of the photomultiplier and acquired the data via an analog-to-digital converter. The algorithms distinguished and separately recorded background glow, intermittent flashes, and total light from populations ranging in number from 10³ to 10⁵ cells in volumes from 1 to 10 ml. Fast video display techniques allowed continuous on-line viewing of incoming data, together with a display of the data recorded over the preceding day or two. Detection of mechanical and software errors coupled with recovery systems maintained high reliability of data collection.

Journal of Biological Rhythms, Vol. 1, No. 3, 251-263 (1986)
DOI: 10.1177/074873048600100307


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