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Photoperiodism in Ostrinia nubilalis: A New Protocol for the Analysis of the Role of the Circadian System

School of Life and Health Sciences, University of Delaware, Newark, Delaware 19716

William Cain

School of Life and Health Sciences, University of Delaware, Newark, Delaware 19716

N.G. Patel

E. I. du Pont de Nemours & Co., Inc., Agricultural Chemicals Department, Experimental Station, Wilmington, Delaware 19898

Dana L. Newswanger

School of Life and Health Sciences, University of Delaware, Newark, Delaware 19716

A 12-hr dark period, at a temperature high enough to permit time measurement to occur, is necessary for maximal induction of larval diapause in the European corn borer, Ostrinia nubilalis. In the present study, induction of diapause only occurred in a periodic environment. This was in the form of certain (1) light-dark (LD) cycles at a constant temperature; (2) thermo periods in constant darkness (DD), but not constant illumination (LL); and (3) LD cycles with concurrent thermoperiods. A light-break experiment protocol, in which the pulses systematically scan the cold and warm phases of a thermoperiod in DD, is discussed as a way of helping clarify how seasonal time measurement is effected in Ostrinia.

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				S. D. Skopik and M. Takeda Diapause Induction and Termination: North-South Strain Differences in Ostrinia nubilalis J Biol Rhythms, March 1, 1987; 2(1): 13 - 22. [Abstract] [PDF]

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